[1]文灿,李淑蓉,张艳玲,等.GDNF及不同生长底物对培养颈上神经节交感神经元的作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):882-884.
 WEN Can,LI Shu-rong,ZHANG Yan-ling,et al.Effects of GDNF and substrate on superior cervical ganglion sympathetic neurons in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(10):882-884.
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GDNF及不同生长底物对培养颈上神经节交感神经元的作用(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第10期
页码:
882-884
栏目:
论著
出版日期:
2007-05-30

文章信息/Info

Title:
Effects of GDNF and substrate on superior cervical ganglion sympathetic neurons in vitro
作者:
文灿李淑蓉张艳玲王晗知苏炳银
第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所;成都医学院:病理学教研室,组织胚胎与神经生物学教研室
Author(s):
WEN Can LI Shu-rong ZHANG Yan-ling WANG Han-zhi SU Bing-yin
Department of Neurobiology, College of Medicine, Third Military Medical University, Chongqing 400038;Department of Pathology,Department of Histology and Embryology and Neurobiology, Chengdu Medical College, Chengdu 610081, China
关键词:
胶质细胞源性神经营养因子生长底物交感神经元
Keywords:
glial cell-derived neurotrophic factor substrate sympathetic neuron
分类号:
R322.85;R329-33;R329.2
文献标志码:
A
摘要:
目的    探讨胶质细胞源性神经营养因子及不同生长底物对培养颈上神经节交感神经元存活和突起生长的影响。    方法    取新生大鼠颈上神经节(superior cervical ganglion, SCG)体外分离培养,培养基中含不同浓度的胶质细胞源性神经营养因子(glial cell derived neurotrophic factor, GDNF),并选取不同方法溶解多聚-D-赖氨酸(poly-D-lysine, PDL)包被培养盖片,观测交感神经元的存活和突起生长状况。    结果    用0.01 mol/L硼酸溶解的PDL进行包被,细胞存活和突起生长最好,且神经元分散好,优于其他各组;GDNF对培养交感神经元的存活、突起生长具有显著的促进作用,且与其浓度存在量效关系,但它并不能阻止培养前5 d存活神经元数量的减少。    结论    用0.01 mol/L硼酸溶解PDL包被是一种良好的生长底物处理方法,GDNF能促进离体培养的交感神经元的存活和突起生长。
Abstract:
Objective    To explore the effects of glial cell-derived neurotrophic factor and different substrates on cell survival and neurite growth of superior cervical ganglion sympathetic neurons from neonatal rat in vitro.     Methods    Sympathetic neurons from superior cervical ganglion (SCG) of neonatal rat respectively were dissociated and cultured. The glial cell-derived neurotrophic factor (GDNF) of different quantities were added in the medium, and culture cover slips were coated by variant methods with poly-D-lysine (PDL). The cell survival and neurite growth of cultured sympathetic neurons were observed by phase-contrast microscopy.     Results    In the group of cover glasses coated with PDL dissolved by 0.01 mol/L boric acid solution, cells grew dispersedly well, and both the cell survival and neurite growth were better than others. GDNF promoted the cell survival and neurite growth of sympathetic neurons markedly,  and there was a dose-effect relationship, but it could not abolish the decrease of survival cells from 1 d to 5 d in vitro.     Conclusion    It is a favorable substrate that coated with PDL dissolved 0.01 mol/L by boric acid solution, and both the cell survival and neurite growth of sympathetic neurons can be improved by GDNF in vitro.

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更新日期/Last Update: 2008-10-24