[1]史淑红,李佃贵,马秀菊.子宫内膜异位症患者腹腔液中血管内皮生长因子及其可溶性受体的测定[J].陆军军医大学学报(原第三军医大学学报),2007,29(10):976-978.
 SHI Shu-hong,LI Dian-gui,MA Xiu-ju.Free vascular endothelial growth factor and its soluble receptor in peritoneal fluid from endometriosis patients[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(10):976-978.
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子宫内膜异位症患者腹腔液中血管内皮生长因子及其可溶性受体的测定
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第10期
页码:
976-978
栏目:
论著
出版日期:
2007-05-30

文章信息/Info

Title:
Free vascular endothelial growth factor and its soluble receptor in peritoneal fluid from endometriosis patients
作者:
史淑红李佃贵马秀菊
河北医科大学第二医院妇产科;河北省中医院内科
Author(s):
SHI Shu-hong LI Dian-gui MA Xiu-ju
Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Department of Internal Medicine, Traditional Chinese Medicine Hospital of Hebei Province, Shijiazhuang 050011, China
关键词:
子宫内膜异位症血管内皮生长因子血管内皮生长因子可溶性受体
Keywords:
endometriosis vascular endothelial growth factor soluble form of VEGFR-1 (s-VEGFR-1/sFlt-1)
分类号:
R392.11; R446.19; R711.71
文献标志码:
A
摘要:
目的    探讨子宫内膜异位症患者血管内皮生长因子可溶性受体1(soluble receptor 1 of vascular endothelial growth factor, s-VEGFR-1/soluble fms-like tyrosine kinase, SFlt-1)调节血管内皮生长因子(vascular endothelial growth factor, VEGF)活动的机制。    方法    采用酶联免疫吸附法(ELISA),对28例子宫内膜异位症患者的腹腔液中游离的VEGF及s-VEGFR-1进行测定并计算活性指数(VEGF/s-VEGFR-1)。以10例正常生育期妇女为对照。    结果    ①子宫内膜异位症组VEGF水平明显高于对照组﹙P<0.01)。Ⅲ~Ⅳ期高于Ⅰ~Ⅱ期﹙P<0.05)。②子宫内膜异位症组s-VEGFR-1水平也明显高于对照组﹙P<0.01)。Ⅰ~Ⅱ期与Ⅲ~Ⅳ期之间无显著差异(P>0.05)。③对照组、Ⅰ~Ⅱ期和Ⅲ~Ⅳ期VEGF活性指数分别为0.310、0.276和0.273。④子宫内膜异位症组增生期VEGF及s-VEGFR-1水平均明显高于分泌期﹙P<0.01)。⑤子宫内膜异位症组增生期VEGF活性指数低于分泌期。    结论    VEGF在子宫内膜异位症的血管发生中有重要作用,但采用VEGF活性指数能更好地了解VEGF在病理学方面的作用。s-VEGFR-1对VEGF有下调作用。
Abstract:
Objective    To better understand the regulation of the soluble receptor of vascular endothelial growth factor (VEGF), s-VEGFR-1 (or soluble fms-like tyrosine kinase, SFlt-1), in angiogenetic process in endometriosis.     Methods    Levels of free VEGF and s-VEGFR-1 were measured by enzyme-linked immunosorbent assay (ELISA) in peritoneal fluid from 28 subjects with surgically confirmed endometriosis, and 10 controls with no clinical evidence of the disease and other diseases. Meanwhile, we calculated a VEGF activity index by means of the ratio VEGF/s-VEGFR-1.     Results    We found higher VEGF concentration in endometriotic lesions than controls (P<0.01) and VEGF concentration in stage Ⅲ-Ⅳ was higher than in stage Ⅰ-Ⅱ (P<0.05). S-VEGFR-1 concentration followed a similar pattern with VEGF (P<0.01), but no statistically significant difference was found between stage Ⅲ-Ⅳ and stage Ⅰ-Ⅱ (P>0.05). VEGF activity index in controls, stage Ⅰ-Ⅱ and stages Ⅲ-Ⅳ of endometriosis was 0.310, 0.276 and 0.273, respectively. VEGF and s-VEGFR-1 concentration were also higher in proliferative phase than in secretory phase in endometriotic lesions (P<0.01). VEGF activity index in proliferative phase was lower than in secretory phase of endometriosis.     Conclusion    VEGF certainly plays an important role in endometriosis and its progression. However, the activity index may be better to investigate the real role of VEGF in the pathology. s-VEGFR-1 can down-regulate VEGF activity.

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更新日期/Last Update: 2008-10-24