[1]王燕,郭乔楠,章波,等.组蛋白乙酰化修饰对IEC-6恶性转化细胞细胞周期和p53、p21WAF1基因表达的调控[J].第三军医大学学报,2007,29(04):300-303.
 WANG Yan,GUO Qiao-nan,ZHANG Bo,et al.Regulation of histone acetylation on cell cycle and p53,p21WAF1 genes expression in malignant transformation of IEC-6[J].J Third Mil Med Univ,2007,29(04):300-303.
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组蛋白乙酰化修饰对IEC-6恶性转化细胞细胞周期和p53、p21WAF1基因表达的调控(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第04期
页码:
300-303
栏目:
论著
出版日期:
2007-02-28

文章信息/Info

Title:
Regulation of histone acetylation on cell cycle and p53,p21WAF1 genes expression in malignant transformation of IEC-6
作者:
王燕郭乔楠章波许雪青白云
第三军医大学:基础医学部医学遗传学教研室,西南医院病理学研究所
Author(s):
WANG Yan GUO Qiao-nan ZHANG Bo XU Xue-qing BAI Yun
Department of Medical Genetics,  College of Medicine, Institute of Pathology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
IEC-6细胞组蛋白乙酰化p53p21WAF1
Keywords:
malignant transformation of IEC-6 histone acetylation  p53 p21WAF1
分类号:
R394.3;R730.2;R735.3
文献标志码:
A
摘要:
目的     分析和探讨IEC-6恶性转化细胞不同时期组蛋白乙酰化修饰对肿瘤发生相关基因表达的影响。     方法     采用我们已建立的3个转化期(化学致癌剂MNNG和诱导剂PMA共同作用6、12、24次) 的IEC-6转化细胞进行培养,提取不同转化时期的IEC-6转化细胞的细胞核蛋白,采用免疫印迹检测H3乙酰化水平;并用ChIP及RT-PCR检测组蛋白H3乙酰化对p21WAF1表达的影响。     结果     24次IEC-6恶性转化细胞组蛋白H3乙酰化水平高于6次IEC-6转化细胞,而组蛋白H3去乙酰化水平低于6次IEC-6转化细胞;同时发现p21WAF1的启动子区PCR产物减少。在6、12次IEC-6转化细胞中均有较弱的p53、p21WAF1表达;在24次IEC-6恶性转化细胞中仍可检测到较弱的p53表达,但p21WAF1表达缺如。     结论     IEC-6恶性转化细胞过程中,组蛋白H3乙酰化水平增高,导致p21WAF1基因表达受抑,近而可能影响转化细胞的细胞周期。
Abstract:
Objective     To investigate the effects of histone acetylation on the expression of p53 and p21WAF1 genes in malignant transformation of IEC-6.      Methods     The malignant transformation of IEC-6 was carried out by self-established three transition phases in which chemical cancerogenic agent MNNG and derivative PMA were used to the target cells for 6, 12, and 24 times. The transformants were cultured and the neucleoprotein of IEC-6 was extracted during every transition phase. The cell cycle distribution was studied by flow cytometry. The level of acetylated histones in chromatin associated with the p53 and p21WAF1 was examined by chromatin immunoprecipitation (ChIP) assay. The expression of p53 and p21WAF1 genes mRNA was detected by RT-PCR.      Results     Acetylation level of histone H3 in IEC-6 was higher after malignant transformation for 24 times than 6 times. Malignant transformation induced an accumulation of acetylated histone H3 in chromatin associated with p21WAF1 gene. Expression of p53 and p21WAF1 was detected weakly in 6 and 12 times malignant transformation of IEC-6. There was no expression of p21WAF1 in malignant transformation of IEC-6 for 24 times.      Conclusion     In the malignant transformation of IEC-6 cell, the expression of p21WAF1 and p53 genes is regulated by histone acetylation. The enhanced acetylation of histone H3 will restrain the expression of p21WAF1 gene and further may affect the cell cycle of transformants.

参考文献/References:

王燕,郭乔楠,章波,等. 组蛋白乙酰化修饰对IEC-6恶性转化细胞细胞周期和p53、p21WAF1基因表达的调控[J]. 第三军医大学学报,2007,29(4):300-303.

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更新日期/Last Update: 2008-11-12