[1]陈莹,钟玲,王勇,等.RNA干扰沉默survivin基因在抑制卵巢癌裸鼠移植瘤中的作用[J].第三军医大学学报,2007,29(06):530-533.
 CHEN Ying,ZHONG Ling,WANG Yong,et al.Effect of RNAi-mediated survivin gene silencing on proliferation of xenograft tumor of ovarian carcinoma[J].J Third Mil Med Univ,2007,29(06):530-533.
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RNA干扰沉默survivin基因在抑制卵巢癌裸鼠移植瘤中的作用(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第06期
页码:
530-533
栏目:
论著
出版日期:
2007-03-30

文章信息/Info

Title:
Effect of RNAi-mediated survivin gene silencing on proliferation of xenograft tumor of ovarian carcinoma
作者:
陈莹钟玲王勇邓凯贤
重庆医科大学附属第二医院妇产科;广东省佛山市妇幼保健院妇科
Author(s):
CHEN Ying ZHONG Ling WANG Yong DENG Kai-xian
Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, Department of Obstetrics and Gynecology, Foshan Maternal and Child Health Hospital, Foshan 528000, China
关键词:
survivin卵巢肿瘤细胞株SKOV3RNA干扰裸鼠
Keywords:
survivin xenograft tumor SKOV3 RNA interference athymic mice
分类号:
R73-362;R730.23;R737.31
文献标志码:
A
摘要:
目的     观察pshRNA-survivin在体内是否能抑制卵巢癌移植瘤体的生长。     方法     建立人卵巢癌裸鼠移植瘤模型,瘤内注射pshRNA-survivin,观察肿瘤生长情况。半定量RT-PCR、免疫组织化学检测移植瘤的survivin表达情况,TUNEL检测移植瘤的细胞凋亡情况。     结果     pshRNA-survivin能明显抑制肿瘤组织中survivin的表达,促进肿瘤细胞的凋亡,减缓肿瘤生长速度。     结论     pshRNA-survivin明显抑制了卵巢癌裸鼠移植瘤的增殖。
Abstract:
Objective     To examine the therapeutic effect of RNAi-mediated survivin gene silencing on the xenograft tumor of ovarian cancer in vivo.      Methods     Subcutaneous tumors in athymic mice were induced by inoculation of SKOV3. pshRNA-survivin and lipofectamine, lipofectamine, pTZU6+1 and lipofectamine, pshRNA survivin, and physical saline were injected into the subcutaneous tumors. The growth of the xenograft tumor was observed after inoculation. The expression of survivin in the xenograft tumor was observed by RT-PCR and immunohistochemistry. The cell apoptosis in tumor tissues was detected by TUNEL.      Results     pshRNA-survivin significantly inhibited survivin expression, accelerated the apoptosis of tumor cells and inhibited the degree and speed of SKOV3 cells progressing into xenograft tumor in nude mice in vivo.       Conclusion     pshRNA-survivin significantly inhibited the proliferation of xenograft tumor of ovarian carcinoma.

参考文献/References:

陈莹,钟玲,王勇,等. RNA干扰沉默survivin基因在抑制卵巢癌裸鼠移植瘤中的作用[J]. 第三军医大学学报,2007,29(6):530-533.

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更新日期/Last Update: 2008-10-29