[1]范东瀛,陈俊英,张新文,等.流感病毒在不同细胞中适宜培养条件的研究[J].第三军医大学学报,2007,29(06):510-512.
 FAN Dong-ying,CHEN Jun-ying,ZHANG Xin-wen,et al.Research on optimal culture condition for influenza virus in different cells[J].J Third Mil Med Univ,2007,29(06):510-512.
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流感病毒在不同细胞中适宜培养条件的研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第06期
页码:
510-512
栏目:
论著
出版日期:
2007-03-30

文章信息/Info

Title:
Research on optimal culture condition for influenza virus in different cells
作者:
范东瀛陈俊英张新文李卫东廖国阳
中国医学科学院中国协和医科大学医学生物学研究所
Author(s):
FAN Dong-ying CHEN Jun-ying ZHANG Xin-wen LI Wei-dong LIAO Guo-yang
Institute of Medical Biology, Chinese Academy of Medical Science, Peking Union Medical College, Kunming 650118, China
关键词:
流感病毒细胞培养血凝滴度
Keywords:
influenza virus cell culture hemagglutination titer
分类号:
Q939.405;R373.13
文献标志码:
A
摘要:
目的     探索流感病毒在细胞中培养的适宜条件。     方法     采用MEK细胞、FRhK-4细胞、MDCK细胞分别接种甲型流感病毒H1N1型、H3N2型和乙型流感病毒疫苗毒株,观察3种毒株在不同培养条件下的细胞病变、血凝滴度。     结果     3株流感病毒接种细胞在33 ℃培养72 h后出现拉网、圆缩、脱落等现象;FRhK-4细胞和MDCK细胞对流感病毒较敏感。胞龄为48 h的细胞,在NaHCO3的终浓度为1.4‰,TPCK-胰酶浓度为1.0 μg/ml的病毒维持液培养流感病毒为最适条件。     结论     寻找到了细胞流感病毒的适宜条件。
Abstract:
Objective     To search for optimal culture condition of influenza virus in the tender cells.      Methods     Influenza A (H3N2, H1N1) and B virus were inoculated in MDCK cells, MEK cells and FRhK-4 cells under different culture conditions to observe cytopathic effect (CPE), hemagglutination titer.      Results     after incubated at 33 ℃ for 72 h, cytopathic effect of viral infection in all three kinds of cells was observed, and hemagglutination assay showed MDCK cells and FRhK-4 cells were more sensitive than MEK cells to influenza virus. It is the best culture condition that the cells aged 48 h in maintenance media containing 1.4‰  NaHCO3, 1.0 μg/ml trypsin-TPCK.      Conclusion     The optimal culture conditions for influenza virus in cells were showed in this study.

参考文献/References:

范东瀛,陈俊英,张新文,等. 流感病毒在不同细胞中适宜培养条件的研究[J]. 第三军医大学学报,2007,29(6):510-512.

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更新日期/Last Update: 2008-10-29