[1]陈琦,彭洪英,杨戎,等.乳酸盆底注射诱导小鼠非细菌性前列腺炎及前列腺上皮内瘤样变[J].第三军医大学学报,2007,29(13):1308-1310.
 CHEN Qi,PENG Hong-ying,YANG Rong,et al.Pelvic floor injection of lactic acid induced histological nonbacterial prostatitis and consequent PIN-like lesion in mice[J].J Third Mil Med Univ,2007,29(13):1308-1310.
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乳酸盆底注射诱导小鼠非细菌性前列腺炎及前列腺上皮内瘤样变(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第13期
页码:
1308-1310
栏目:
论著
出版日期:
2007-07-15

文章信息/Info

Title:
Pelvic floor injection of lactic acid induced histological nonbacterial prostatitis and consequent PIN-like lesion in mice
作者:
陈琦彭洪英杨戎魏莎莉王德林谭毅
重庆医科大学:实验动物中心,生殖生理学教研室,附属第一医院泌尿外科
Author(s):
CHEN QiPENG Hong-ying YANG RongWEI Sha-liWANG De-linTAN Yi
Laboratory Animal Center, Division of Reproduction Physiology, Department of Urology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
关键词:
乳酸非细菌性前列腺炎前列腺上皮内瘤
Keywords:
lactic acid nonbacterial prostatitis PIN
分类号:
R363.13; R697.33
文献标志码:
A
摘要:
目的    乳酸的盆底聚集可能在慢性非细菌性前列腺炎发病过程中起着始动作用,采用盆底注射乳酸诱导小鼠前列腺组织的炎症改变加以验证。    方法    对小鼠进行第1、2、4、7天多次盆底乳酸注射诱发前列腺炎症,并于第2、3、5、8天摘取前列腺组织制作组织学标本,进行HE染色观察。    结果    组织切片反映出前列腺组织按照盆底乳酸注射时间的延长依次出现了以间质充血水肿,间质及腺泡周围炎性浸润,腺泡腔内出现炎性分泌物为代表特征的炎症病变,最后一组标本中除炎症反应外还观察到了较为明显的腺泡上皮增生,类似于人类的前列腺上皮内瘤(prostatic intra-epithelial neoplasia, PIN)样变。    结论    经乳酸盆底注射诱导的小鼠前列腺炎症改变类似于临床患者的病理表现,提示乳酸的盆底聚集在慢性前列腺炎发病机制中起到了重要作用;本实验中观察到的前列腺炎症后PIN样改变为研究慢性前列腺炎与前列腺癌前病变的发生提供了良好的动物模型。
Abstract:
Objective    Lactic acid is supposed to trigger the process of chronic nonbacterial prostatitis. To prove this hypothesis, lactic acid was introduced as an irritating agent to induce mice’s histological nonbacterial prostatitis by pelvic floor injection.     Methods    Adolescent mice received repeated injections of lactic acid (0.03 mol/L, 0.1 ml per time) on the 1st, 2nd, 4th, 7th days. Each of them was sacrificed on the second day after their last injection. All dissected prostates were fixed in formalin and then sectioned for HE staining.     Results    The prostates undergoing serials of inflammatory changes sequenced as: edema and venous dilation in the stroma, lymphocytes infiltration of the stroma and periglandular region, inflammatory exudates within the acinus. Besides, distinctive epithelial proliferation change was observed on the 8thday which is basically similar to PIN-like lesion in human prostate.     Conclusion    Lactic acid induced histological prostatitis replicates the pathological changes observed in clinical patients, suggesting the aggregation of lactic acid in the pelvic region plays an important role in the pathogenesis of chronic nonbacterial prostatitis. This study also supports the association between chronic prostatitis and later prostate cancer in a mouse model.

参考文献/References:

陈琦, 彭洪英, 杨戎, 等. 乳酸盆底注射诱导小鼠非细菌性前列腺炎及前列腺上皮内瘤样变[J]. 第三军医大学学报, 2007, 29(13):1308-1310.

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更新日期/Last Update: 2008-10-15