[1]郑鸿燕,邰浩清,周春祥,等.蒺藜皂苷诱导SD新生大鼠海马神经干细胞分化的实验研究[J].第三军医大学学报,2007,29(18):1764-1767.
 ZHENG Hong-yan,TAI Hao-qing,ZHOU Chun-xiang,et al.Effects of total saponins of tribulus on the differentiation of rat neural hippocampus stem cells[J].J Third Mil Med Univ,2007,29(18):1764-1767.
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蒺藜皂苷诱导SD新生大鼠海马神经干细胞分化的实验研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第18期
页码:
1764-1767
栏目:
论著
出版日期:
2007-09-30

文章信息/Info

Title:
Effects of total saponins of tribulus on the differentiation of rat neural hippocampus stem cells
作者:
郑鸿燕邰浩清周春祥吴颢昕
南京中医药大学解剖学教研室
Author(s):
ZHENG Hong-yan TAI Hao-qing ZHOU Chun-xiang WU Hao-xin
Department of Anatomy, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China
关键词:
蒺藜皂苷海马NSCs神经元神经胶质细胞
Keywords:
total saponins of tribulus neural hippocampus stem cells neuron astrocyte
分类号:
R285.5;R322.81;R329.28
文献标志码:
A
摘要:
目的  探讨蒺藜皂苷对于诱导SD新生大鼠海马神经干细胞(neural stem cells, NSCs)分化的影响作用以及蒺藜皂苷培养液浓度变化对其的影响。 方法  采用无血清和单克隆培养方法,将蒺藜皂苷加入离体的海马NSCs的培养液中,应用Nestin、BrdU、NF200、GFAP免疫荧光染色观察NSCs的形态及分化而来的神经元、神经胶质细胞形态,并检测由海马NSCs分化而来的细胞的数量。 结果  海马NSCs在无血清培养下,可形成Nestin阳性细胞球。在诱导分化后,免疫荧光染色可见NF阳性细胞、GFAP阳性细胞,而其中20 mg/kg 蒺藜皂苷干预组海马NSCs向神经元的分化比例最高。 结论  在蒺藜皂苷的作用下,海马NSCs向神经元分化的数量增多。
Abstract:
Objective    To observe the effects of total saponins of tribulus of various consistency on the differentiation of neural hippocampus stem cells.     Methods    The hippocampus stem cells from newborn rat were obtained by serum-free medium and single-cell cloning technique. Hippocampus stem cells were induced by total saponins of tribulus of various consistency. Nestin, BrdU, neurofilament (NF-200), glial fibrillary acidic protein (GFAP) expression were detected using immunocytochemical stains. The numbers of NF and GFAP positive cells were counted. The percentages of positive cells were obtained.     Results    The dissociated cells from the organ of hippocampus developed into much of indiffrentiated otopheres of nestin positive cells. The hippocampus stem cell differentiated into neurons and astrocytes with the total saponins of tribulus. The percentage of NF positive neuron in the 20 mg/kg consistency of total saponins of tribulus groups was significantly higher than that in the control group and other consistency of total saponins of tribulus groups.     Conclusion    Total saponins of tribulus may enhance neural hippocampus stem cells differentiate into NF positive neuron.

参考文献/References:

郑鸿燕,邰浩清,周春祥,等.  蒺藜皂苷诱导SD新生大鼠海马神经干细胞分化的实验研究[J].第三军医大学学报,2007,29(18):1764-1767.

更新日期/Last Update: 2008-07-16