[1]叶兰,徐晓玉,李荣亨,等.三棱、莪术含药血清对培养的人脐静脉血管内皮细胞生长和VEGF表达的影响[J].第三军医大学学报,2007,29(02):121-124.
 YE Lan,XU Xiao-yu,LI Rong-heng,et al.Effect of medicated serum of Sangleng and Eshu on human umbilical vein vascular endothelial growth factor and vascular endothelial cells[J].J Third Mil Med Univ,2007,29(02):121-124.
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三棱、莪术含药血清对培养的人脐静脉血管内皮细胞生长和VEGF表达的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第02期
页码:
121-124
栏目:
论著
出版日期:
2007-01-30

文章信息/Info

Title:
Effect of medicated serum of Sangleng and Eshu on human umbilical vein vascular endothelial growth factor and vascular endothelial cells
作者:
叶兰徐晓玉李荣亨何正光陈刚
贵阳医学院药理学教研室;西南大学药学与中医药学院; 重庆医科大学: 附属第一医院中医科,药学院;重庆工商大学药物化学与化学生物学研究中心
Author(s):
YE Lan XU Xiao-yu LI Rong-heng HE Zheng-guang CHEN Gang
Department of Pharmacy,  Guiyang Medical College, Guiyang 550004; College of Chinese Traditional Medicine and Pharmacy, Southwest University, Chongqing 400715;Department of Traditional Medicine, The First Affiliated Hospital, College of Pharmacy, Chongqing Medical University, Chongqing 400016; Research Center of Pharmaceutical Chemistry & Chemobiology, Chongqing Technology and Business University, Chongqing 400067, China
关键词:
三棱莪术含药血清人脐静脉血管内皮细胞VEGF
Keywords:
Sanleng Eshu medicated serum human umbilical vein endothelial cell vascular endothelial growth factor
分类号:
R285.5;R322.123;R392.11
文献标志码:
A
摘要:
目的     探讨三棱、莪术含药血清对血管内皮生长因子(vascular endothelial growth factor, VEGF)诱导的血管内皮细胞增殖和VEGF表达影响。     方法     以三棱、莪术含药血清作用于VEGF诱导的人脐静脉血管内皮细胞(human umbilical vein endothelial cell-1, HUVEC-1),倒置相差显微镜观察细胞形态学改变,MTT法观察细胞增殖情况, Western blot和RT-PCR方法分别检测内皮细胞VEGF蛋白和mRNA的表达。     结果     三棱、莪术5.0 g·kg-1·d-1、2.5 g·kg-1·d-1大鼠含药血清可使正常人脐静脉血管内皮细胞排列紊乱,明显梭形化。5.0 g·kg-1·d-1大鼠10%、5%、2.5%含药血清组和2.5 g·kg-1·d-1大鼠10%含药血清组HUVEC-1细胞的增殖显著低于空白血清相同浓度组(P<0.05)。5.0 g·kg-1·d-1、2.5 g·kg-1·d-1大鼠5%的含药血清使HUVEC-1细胞VEGF蛋白、VEGF mRNA表达均显著降低。     结论     三棱、莪术含药血清可抑制VEGF诱导的血管内皮细胞增殖,其机制与抑制血管内皮生长因子表达密切相关。
Abstract:
Objective     To explore the effect of medicated serum of Sangleng and Eshu on the expression of vascular endothelial growth factor and vascular endothelial cell proliferation induced by VEGF in vitro.      Methods     Medicated serum of Sangleng and Eshu was used to culture human umbilical vein endothelial cell (HUVEC-1) induced by VEGF. The morphologic changes of HUVEC-1 were observed with phase contrast microscope, and cell proliferation was detected by MTT method, and the expression of vascular endothelial growth factor protein and mRNA in endothelial cells was detected by Western blotting and RT-PCR.      Results     The medicated serum of 5.0, 2.5  g·kg-1·d-1 Sangleng and Eshu could cause arrangement disorder in the normal umbilical vein endothelial cells. The medicated serum of 5.0 g·kg-1·d-1 Sangleng and Eshu (10%, 5%, 2.5%) and medicated serum of 2.5  g·kg-1·d-1 (10%) could inhibit vascular endothelial cell proliferation remarkably (P<0.05), as compared with the blank serum of identical concentration. The medicated serum of 5 g·kg-1·d-1 and 2.5  g·kg-1·d-1 (5%) could reduce significantly the expression of vascular endothelial growth factor protein and mRNA of HUVEC-1.      Conclusion     The medicated serum of Sangleng and Eshu could inhibit vascular endothelial cell proliferation induced by VEGF in vitro, and its mechanism might be associated with inhibited expression of vascular endothelial growth factor.

参考文献/References:

叶兰,徐晓玉,李荣亨,等. 三棱、莪术含药血清对培养的人脐静脉血管内皮细胞生长和VEGF表达的影响[J]. 第三军医大学学报,2007,29(2):121-124.

更新日期/Last Update: 2008-11-13