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Long-term intermittent cold exposure regulates glucose homeostasis via intervening metabolism of adipose tissue in mice
 

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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

Issue:
2019年第11期
Page:
1044-1051
Research Field:
基础医学
Publishing date:

Info

Title:

Long-term intermittent cold exposure regulates glucose homeostasis via intervening metabolism of adipose tissue in mice
 

Author(s):

ZHOU Xin WANG Lan ZHANG Chenliang LIN Shu SONG Zhiyuan

Department of Cardiology, Chongqing Institute of Interventional Cardiology, First Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400038, China

Keywords:

glucose metabolism cold exposure brown adipose tissue browning of white fat adipose transcriptome profiling analysis

PACS:
Q591.4; Q591.5; R339.55
DOI:
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Abstract:

Objective By stimulating intermittent cold exposure to mice for a long term, to investigate the effect of cold exposure on amelioration of glucose homeostasis and prevention of glucose homeostasis disorder induced by high-fat diet (HFD), and analyze the regulative effect of adipose tissue in the process. MethodsA total of 24 male C57 BL/6J male mice (8 weeks old) were equally divided into 4 groups, that is, control group (normal diet+room temperature), cold exposure group (normal diet+4 ℃, 2 h/d), HFD group (HFD feeding+room temperature), and HFD and cold exposure group. Intraperitoneal glucose tolerance test (IPGTT) and insulin tolerance test (IPITT) were carried out in 1, 8, 16 and 22 weeks after intervention. In 22 weeks after intervention, scapular brown adipose tissue (BAT) and inguinal white adipose tissue (WATi) were collected for transcriptome profiling analysis. ResultsWhen having no effect on the bodyweight, intermittent cold exposure for 22 weeks significantly reduced the weight of WATi (0.221±0.016 vs 0.338±0.024 g, P<0.05), but increased the weight of BAT (0.095±0.005 vs 0.079±0.003 g, P<0.05) when compared with the control group, and elevated glucose tolerance by 25.9% (P<0.01) and enhanced insulin sensitivity by 50.4% (P<0.01). The amelioration of glucose homeostasis was positively correlated with the length of cold exposure. However, in the mice with HFD feeding, the cold exposure inhibited the increase of bodyweight (43.3±1.8 vs 32.9±0.7 g, P<0.01) and WATi (1.186±0.215 vs 0.434±0.059 g, P<0.05), and improved insufficient glucose tolerance by 25.5% (P<0.01) and insulin resistance by 33.9% (P<0.01) when compared to the mice of HFD and cold exposure group. Transcriptome profiling analysis showed that long-term intermittent cold exposure obviously activated the glycolysis in the brown fat and white fat tissues, and the PI3K-Akt and insulin signaling pathways (P<0.01), but HFD reversed the activation of glycolysis-related pathways in white fat tissues. Conclusion Long-term intermittent cold exposure may promote glucose homeostasis and prevent glucose homeostasis disorder induced by HFD via activating glycolysis, PI3K-Akt and insulin signaling pathways in adipose tissue.
 

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Last Update: 2019-06-06