|Table of Contents|

Budesonide suppresses nasal mucosal remodeling by regulating IL-4/IL-13/STAT6 pathway in rats with allergic rhinitis

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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

Issue:
2018年第12期
Page:
1085-1090
Research Field:
基础医学
Publishing date:

Info

Title:

Budesonide suppresses nasal mucosal remodeling by regulating IL-4/IL-13/STAT6 pathway in rats with allergic rhinitis

Author(s):

budesonide; allergic rhinitis; interleukin-4; interleukin-13; STAT6; liquid chip; rats

Keywords:
 
PACS:
R765.21;R967;R987
DOI:
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Abstract:

ObjectiveTo investigate the effect of budesonide on nasal mucosal remodeling and the role of IL4/IL13/STAT6 signaling pathway in mediating this effect in rats with allergic rhinitis (AR). MethodsA total of 102 rats were randomly allocated into 3 equal groups, namely ARPBS group, ARbudesonide group and blank group. The rats in ARPBS and ARbudesonide groups were sensitized by challenges with ovalbumin to establish models of AR, and were subsequently treated by daily intranasal instillation with PBS and budesonide with aid of Eppendof for 7 consecutive days, respectively. The rats in the blank group were not challenged with ovalbumin and were treated with PBS in the same manner. Animal Assessment Scale was used to assess the behavioral changes of the rats after the treatments. Enzymelinked immunosorbent assay (ELISA) was used to detect the serum levels of histamine (His) and IgE, and the levels of interleukin (IL)13 and IL4 in the serum and bronchoalveolar lavage were analyzed with a Luminex liquid chip. STAT6 mRNA expression in the nasal mucosal was assessed with qPCR, and the protein expression levels of STAT6 and PSTAT6 were tested with Western blotting. HE staining and transmission electron microscopy were used to assess nasal septum remodeling after the treatments. ResultsThe rat models of AR all had a behavioral assessment score over 5 by the Animal Assessment Scale. Compared with those in the blank group, the rats with AR showed significantly increased serum levels of His and IgE, increased levels of IL13 and IL4 in both the serum and bronchoalveolar lavage, and also increased expression of STAT6 and PSTAT6 in the nasal mucosa (P<0.01). Budesonide treatment of the rats with AR for 7 days obviously lowered the serum levels of His and IgE, which remained unchanged in rats with PBS treatment. Compared with PBS treatment, budesonide treatment also decreased the levels of IL4 and IL13 in the serum and bronchoalveolar lavage and downregulated the expression of STAT6 and PSTAT6 in the nasal mucosa (P<0.01). HE staining and transmission electron microscopy showed that budesonide treatment partially reversed ARinduced reduction of nasal cilia in the rats without causing obvious changes in cell connection. ConclusionBudesonide improves the pathologies in the nasal mucosa of rats with AR by inhibiting IL4, IL13, and pSTAT6.

References:

 
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Last Update: 2018-07-03