|Table of Contents|

Comparison of demineralization kinetic curves of 2 commonly used demineralizing solutions and their effects on collagen dissolution and degradation before dentin bonding

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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

Issue:
2018年第12期
Page:
1155-1160
Research Field:
生物医学工程
Publishing date:

Info

Title:

Comparison of demineralization kinetic curves of 2 commonly used demineralizing solutions and their effects on collagen dissolution and degradation before dentin bonding

Author(s):

KOU Tingting XIONG Yu CUI Yue CAI Jiangwen ZHU Can ZHOU Jixiang

Department of Stomatology, First Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400038, China
 

Keywords:

acid etching demineralization collagenase kinetic curves dentin
 

PACS:
R313;R783.1;R988.2
DOI:
-
Abstract:

ObjectiveTo compare the demineralization efficiency of 37% phosphoric acid and 17% EDTA in human dentin and evaluate the effect of different etching time on the degradation rate of type Ⅰ collagen matrix in human dentin. MethodsEqual amounts of human dentin powder were demineralized with 37% phosphoric acid and 17% EDTA for 0 s (control group), 10, 15, 30, 60 and 120 s, or 72 h. After centrifugation and washing, the samples were analyzed for concentrations of Ca2+, Mg2+, and P5 in the washing solution using an automatic biochemical analyzer. The demineralization curves were drawn and the demineralization efficiency of the 2 demineralization solutions was compared. The precipitates in each group were enzymatically hydrolyzed on day 1 and day 7 with 300 μL type Ⅰ collagenase solution (1 g/L). Pearson correlation coefficient was used to analyze the correlation among the total demineralization, collagen dissolution and collagen degradation. ResultsTreatment with 37% phosphoric acid solution for 15 s caused obvious demineralization in the dentin samples, and collagen dissolution increased significantly at 30 s (P<0.05); collagen degradation levels did not show significant changes over time, and the increment of collagen degradation began to decrease significantly at 15 s (P<0.05). The amount pf demineralization was strongly correlated with collagen dissolution (r=0.864, P<0.05) and degradation increment (r=-0.713, P<0.05). Treatment with 17% EDTA for 30 s resulted in obvious demineralization of dentin but less obvious collagen dissolution, which showed no significant changes within 120 s of treatment (P<0.05); The peak level of collagen degradation occurred at 30 s followed by significant and progressive decreases (P<0.05), and the degradation increment began to decrease significantly at 60 s (P<0.05). The amount of demineralization was strongly correlated with collagen dissolution (r=0.894, P<0.05). ConclusionPhosphoric acid results in rapid and efficient demineralization of dentin with a high collagen dissolution rate, and the dentin collagen matrix has a good stability after demineralization. EDTA has a relatively low efficiency of demineralization and collagen dissolution, and the stability of dentin collagen matrix after demineralization is acceptable. Clinically, both 37% phosphoric acid with an etching time of 10~15 s and 17% EDTA acid with an etching time of 60~90 s can be optimal for dentin treatment before dentin bonding.

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Last Update: 2018-07-03