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Genomic analysis of vaginal microbiome community in female early rheumatoid arthritis patients



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Genomic analysis of vaginal microbiome community in female early rheumatoid arthritis patients


HUI Caixia ZHANG Juan ZENG Benhua ZHONG Bing WAN Ping YANG Li WU Like WEI Hong FANG Yongfei

Department of Integrated Chinese and Western Medicine, 2Institute of Infectious Diseases, First Affiliated Hospital; 3Department of Laboratory Animal Science, College of Basic Medical Sciences, Army Military Medical University (Third Military Medical University), Chongqing, 400038, China


rheumatoid arthritis vaginal microbiome highthroughput sequencing

R322.65; R372; R593.22

Objective    To analyze the differences of vaginal microbiome between the patients with early rheumatoid arthritis (RA) and healthy reproductive-age women from the overall perspective of bacteria.   Methods    A total of 24 vaginal swabs were collected from 12 early RA patients and 12 healthy women (control). The bacterial genomic DNA of all obtained samples was extracted for amplifying the 16S rRNA V3 and V4 hypervariable region by PCR and pyrosequencing by Illumina. The abundance and composition of vaginal microbiota were analyzed by Uparse and LEfSe software. Results    The Alpha diversity was significantly increased in the bacterial strains from the early RA patients (P=0.04). Lactobacillus was the predominant genus in healthy women (accounting for 84.98%). The amount of Lactobacillus was decreased (11.01%) but that of Gardnerella increased (43.16%) significantly in the RA group. Moreover, the relative abundances of Bifidobacterium (9.94%), Streptococcus (8.05%) and Atopobium (7.86%) were increased in different degrees. The results of principal coordinate analysis (PCoA) and hierarchical cluster analysis showed that the structure of vaginal flora in RA group had high similarity, and the vaginal microflora structure of the healthy control group could be distinctly distinguished. LEfSe analysis found that there were significant differences in the abundance of Lactobacillus, Gardiner, bifidobacteria and Singularities at the genus level (P=0.017, 0.046, 0.018, 0.007).  Conclusion    A correlation is found between vaginal dysbacteriosis and RA. The diversity of vaginal microbiome is increased in the RA patients, with significantly decreased relative abundance of Lactobacillus, while increased abundance of Gardnerella.


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Last Update: 2018-06-14