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Effect of short-term large-dose exposure to traffic-related PM2.5 from Chongqing, China on lung inflammation in rats 



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Effect of short-term large-dose exposure to traffic-related PM2.5 from Chongqing, China on lung inflammation in rats 


DING Huanyu DUAN Chunyan GAO Jiangyuan LIU Fangxin

College of Medical Technology, Chongqing Medical and Pharmaceutical College, Chongqing, 401331; Experimental Teaching Center,  Chongqing Medical University, Chongqing, 400016, China


PM2.5 rats inflammation cytokine

ObjectiveTo investigate the effects of shortterm highdose exposure to trafficrelated PM2.5 on the expression of inflammatory factors in the lung tissues of rats. MethodsPM2.5 samples were collected at urban roadside locations with a heavy traffic load in Chongqing from September, 2016 to February, 2017 and prepared into PM2.5 suspension. Forty healthy female SD rats were randomly allocated into 2 equal groups for daily exposure (Animal wholebody exposure device, Tianjin Hepu 8050CFA) to aerosol of 0.2 mg/mL PM2.5 suspension (atomizing velocity 2.5 mL/min, intake flow rate 1 m3/min, for 2 h per day) for 17 consecutive days (exposure group,  n=20) or with no treatment (control group,  n=20). The rats were sacrificed at the end of the exposure, and bronchoalveolar lavage fluid (BALF) was collected for measurement of the levels of interleukin1β (IL1β), IL4, IL6 and tumor necrosis factorα (TNFα) using enzymelinked immunosorbent assay (ELISA). The expression of IL1β, IL4, IL6 and TNFα at mRNA level in the lung tissue of the rats were detected using qPCR, and pathological changes in the lungs were examined. ResultsThe rats with exposure to trafficrelated PM2.5 showed a significant reduction in body weight as compared with the control rats (229.26±8.14 g vs 268.65±9.37 g, P<0.05). PM2.5 exposure resulted in significantly increased counts of total inflammatory cells [(931.10±52.94)×106/L], neutrophils [(148.65±23.13)×106/L], monocytes/macrophages [(232.35±21.81)×106/L] and lymphocytes [(547.20±31.18)×106/L] and elevated levels of IL1β [(118.13±10.01) pg/mL], IL4 [(81.65±5.63) pg/mL], IL6 [(135.97±4.93) pg/mL], and TNFα (127.17±9.77) pg/mL in the BALF compared with control group (P<0.05). The exposure also caused significantly increased mRNA expression of IL1β (3.06±0.16), IL4 (17.35±0.54) and IL6 (7.88±1.01) and lowered expression of TNFα mRNA (0.83±0.56) in the lung tissue (P<0.05). Pathological examination revealed massive lung inflammatory cell infiltration in the lungs of the exposed rats. ConclusionShortterm highdose exposure to trafficrelated PM2.5 induces obvious inflammation in the lungs of rats.


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Last Update: 2018-07-03