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Interaction between 5-hydroxytryptamine receptor 1B gene and serotonin transporter gene is potentially associated with suicide attempts in youths



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Interaction between 5-hydroxytryptamine receptor 1B gene and serotonin transporter gene is potentially associated with suicide attempts in youths


LIU Wei RAN Liuyi JIN Jiajia WANG Suya WU Tong AI Ming CHEN Jianmei KUANG Li

Center for Mental Health Services, University-Town Hospital of Chongqing Medical University, Chongqing, 401331; 2Center for Mental Health Services, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China


youths attempted suicide gene-gene interaction 5-hydroxytryptamine receptor 1B 5-hydroxytryptamine transporter


ObjectiveTo explore the interaction between 5-hydroxytryptamine (5-HT) receptor 1B (5-HTR1B) gene and 5HT transporter (5-HTT) gene in the 5-HT system and investigate how this interaction is associated with attempted suicide in youths. MethodsiMLDRTM multiplex single nucleotide polymorphism (SNP) typing was used to examine the SNPs at one locus (rs6296) of 5HTR1B gene and 3 loci (rs1042173, rs140701 and rs2066713) of 5-HTT gene in 70 youths (aged 15-24 years) with attempted suicide (case group) and 112 age-matched healthy subjects (control group). The interaction between 5-HTR1B gene and 5HTT gene was analyzed using generalized multifactor dimensionality reduction (GMDR). ResultsNo significant differences were found between the case and control groups in the genotype frequencies or allele frequencies at the loci rs1042173, rs140701, or rs2066713 in 5HTT gene (P>0.05). The allele frequency of rs6296 in 5HTR1B gene was similar between the 2 groups (P>0.05), but the CC, CG, and GG genotype frequencies of 5HTR1B rs6296 differed significantly between the case group (31.4%, 31.4%, and 37.1%, respectively) and the control group (19.8%, 53.2%, and 27%, respectively) (Chi-square=8.327, P=0.016); these differences, however, were not statistically significant after Bonferroni correction (P>0.05). GMDR analysis indicated the presence of interactions between 5-HTT gene and 5-HTR1B gene in the two-site model (rs1042173 and rs6296) with a test accuracy of 0.6185 and  a cross validation consistency of 10/10 (P=0.021 0-0.022 0), in the three-site model (rs1042173, rs140701, and rs6296) with a test accuracy of 0.644 5 and cross validation consistency of 10/10 (P=0.0030-0.0040) as well as in the four-site model (rs1042173, rs140701, rs2066713, and rs6296) with a test accuracy of 0.6120 and a cross validation consistency of 10/10 (P=0.0320-0.0330). ConclusionThere are interactions between 5-HTR1B gene and 5-HTT gene, which may be associated with attempted suicide in youths.


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Last Update: 2018-07-03