|Table of Contents|

Smad signaling pathway regulates bone morphogenetic protein 9induced osteogenic differentiation of rat dental follicle stem cells in vitro

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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

Issue:
2018年第12期
Page:
1066-1072
Research Field:
基础医学
Publishing date:

Info

Title:

Smad signaling pathway regulates bone morphogenetic protein 9induced osteogenic differentiation of rat dental follicle stem cells in vitro

Author(s):

REN Ge NIE Li YANG Xia HUANG Lina WANG Ming QIU Ye LI Jieyi LI Conghua

Chongqing Key Laboratory of Oral Disease and Biomedical Sciences, Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Stomatological Hospital of Chongqing Medical University, Chongqing, 401147,China

Keywords:

rat dental follicle stem cells bone morphogenetic protein 9 osteogenesis   Smad signaling pathway

PACS:
R322.71;R329.2;R394.2
DOI:
-
Abstract:

ObjectiveTo investigate the role of Smad signaling pathway in the regulatory mechanism of bone morphogenetic protein 9 (BMP9)induced osteogenic differentiation of rat dental follicle stem cells (rDFCs). MethodsThe third passage of purified rDFCs were transfected with the recombinant adenoviruses expressing BMP9 (AdBMP9), and the early and late osteogenesis of rDFCs was detected using realtime qPCR, alkaline phosphatase (ALP) staining, ALP activity assay and Alizarin red S staining. The phosphorylation of Smad1/5/8 in the transfected cells was determined using Western blotting. ResultsCompared with the control cells, AdBMP9transfected rDFCs showed significantly enhanced expression of the osteogenic factors including Runx2, Osterix, ALP and osteopontin(OPN) (P<0.05) with also increased calcium deposition and formation of calcium nodules in the early, intermediate and late stages of osteogenesis. Adenovirusmediated BMP9 gene transduction significantly increased the phosphorylation levels of Smad1/5/8 proteins in rDFCs. ConclusionBMP9 can promote the osteogenic differentiation of rDFCs by activating Smad signaling pathway and increasing the phosphorylation level of Smad1/5/8 proteins to enhance the expression of ALP and osteogenic factors in the early, intermediate and late stages of differentiation.

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Last Update: 2018-07-02