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COMMD7 inhibits migration and invasion in liver cancer stem cells via regulating mesenchymalepithelial transition



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COMMD7 inhibits migration and invasion in liver cancer stem cells via regulating mesenchymalepithelial transition


TAN Ye YOU Nan ZHENG Lu HUANG Xiaobing WANG Liang WU Ke DENG Changlin LI Jing

Department of Hepatobiliary Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing, 400042, China


COMMD7 liver cancer stem cells invasion and migration

R394.3; R730-37; R735.7

Objective     To determine the effect of COMMD7 inhibition on invasion and migration in liver cancer stem cells (LCSCs), and investigate the possible mechanism. Methods    After LCSCs were infected by shRNA lentiviral vectors of COMMD7, adhesion assay and Transwell assay were used to detect  the invasion and migration, and phalloidin staining was employed to observe the morphological changes. Western blotting was adopted to measure the expression of E-cadherin, N-cadherin and Vimentin. Results     COMMD7 knockdown significantly inhibited the invasion and migration of LCSCs. The relative cell quantity of adhesion was 1.00±0.12 and 2.35±0.20 respectively in control cells and infected cells, suggesting there were significantly more adhesive cells in the infected group (P<0.05). The relative cell quantity per visual field of migration was 1.00±0.04 and 0.24±0.03, and that of invasion was 1.00±0.05 and 0.24±0.04 respectively in the control cells and infected cells, and there were significantly less invasive and migrated cells in the infected group (P<0.05). What’s more, COMMD7 knockdown also induced some morphological changes of cells corresponding to the weakened abilities of migration and invasion. All the changes above were associated with up-regulation of E-cadherin (P<0.05) and down-regulation of N-cadherin and Vimentin (P<0.05), the molecules related to mesenchymal-epithelial transition (MET). Conclusion    COMMD7 knockdown inhibits the invasion and migration in LCSCs, which may be through its regulation on the MET course.


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Last Update: 2017-09-01