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Up-regulation of miRNA-671 inhibits proliferation and invasion of gastric cancer cells



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Up-regulation of miRNA-671 inhibits proliferation and invasion of gastric cancer cells


TANG Jiajia YE Lin

Department of Critical Care Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China


miR-671 gastric cancer proliferation migration


Objective      To investigate the expression of miR-671 in gastric cancer tissues and cell lines, and determine its effects on the proliferation and invasion of gastric cancer cells. Methods     The expression of miR-671 was detected in gastric cancer tissues and para-carcerous tissues from 8 patients and cell lines SGC7901, MKN28 and MKN45, and human gastric mucosal epithelial cell line GES-1 by quantitative real-time PCR (qRT-PCR). CCK-8 assay was used to measure the cell proliferation after miR-671 up-regulation. The effect of miR-671 on cell migration and invasion was detected by wound healing assay and Transwell chamber assay. Results     The expression level of miR-671 was significantly decreased in gastric cancer tissues than the adjacent normal tissues (P<0.01). The expression levels of miR671 were significantly decreased in the gastric cancer cell lines compared with GES-1 cells (P<0.01). Up-regulation of miR-671 significantly inhibited the proliferation, migration and invasion in gastric cancer cells (P<0.01). Conclusion     miR-671 inhibits proliferation, migration and invasion of gastric cancer cells, and may serve as a tumor suppressor.


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Last Update: 2017-08-23