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STYK1 down-regulation suppresses colorectal cancer cell growth and invasion in vitro



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STYK1 down-regulation suppresses colorectal cancer cell growth and invasion in vitro


TANG Jiajia YE Lin

Department of Critical Care Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China


serine/threonine/tyrosine kinase 1 colorectal cancer proliferation invasion

R394.3; R730.23; R735.3

Objective     To determine the effects of down-regulating serine/threonine/tyrosine kinase 1 (STYK1) on the proliferation, apoptosis and invasion and on MEK/ERK and PI3K/AKT signaling pathways in human colorectal cancer cell line HCT-15. Methods     The expression of STYK1 protein was down-regulated by RNA interference technique in HCT-15 cells. Then, the STYK1 protein expression was detected by Western blotting. CCK-8 assay was used to measure the effect of STYK1 downregulation on the proliferation of the cells, flow cytometry was employed to detect the cell apoptosis, and Transwell chamber assay was adopted for cell invasion ability. The expression levels of pERK1/2 and p-AKT were detected by Western blotting. Results   Compared with the siRNA NC group, STYK1 down-regulation significantly inhibited the proliferation (P<0.01) and invasion (P<0.01), induced cell apoptosis (P<0.01), and suppressed the expression levels of p-ERK1/2 and p-AKT in the HCT cells transfected with STYK1 siRNA (P<0.01). Conclusion     STYK1 downregulation inhibits the proliferation and invasion and induces the apoptosis in colorectal cancer cells, which may be related to its inhibition on the MEK/ERK and PI3K/AKT signaling pathways.


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Last Update: 2017-09-04