|Table of Contents|

Dihydromyricetin improves cardiac function in ob/ob mice and its underlying mechanism

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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

Issue:
2017年第16期
Page:
1612-1617
Research Field:
基础医学
Publishing date:

Info

Title:

Dihydromyricetin improves cardiac function in ob/ob mice and its underlying mechanism

Author(s):

GONG Xinhua ZHOU Qicheng GU Yeyun ZHU Jundong MI Mantian

Department of Nutrition and Food Hygiene, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing Center of Medical Nutrition, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038, China

Keywords:

dihydromyricetin insulin resistance cardiac function peroxisome proliferator-activated receptor gamma coactivator 1-alpha fibronectin typeⅢdomain-containing protein 5 ob/ob mice

PACS:
R151.3; R331.31; R589.2
DOI:
-
Abstract:

Objective       To determine the protective effect of dihydromyricetin (DHM) on cardiac function in ob/ob mice (with leptin mutation) and investigate the underlying mechanism. Methods      Thirty-six male ob/ob mice were randomly divided into 3 groups: ob/ob group, ob/ob+DHM 50 mg/kg group, ob/ob+DHM 100 mg/kg group (n=12). Ten C57BL/6J wild type mice are used as normal control (WT). Glucose tolerance and insulin tolerance were measured after 16 weeks’ treatment. Cardiac function was detected by echocardiography. Myocardial histopathology and collagen deposition were detected by HE and Masson staining. The protein expression levels of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and fibronectin typeⅢ domain-containing protein 5 (FNDC5) in the myocardium were detected by Western blotting. The expression of FNDC5 in the myocardium was observed by immunohistochemical assay. Results       Glucose tolerance and insulin tolerance were obviously injured in the ob/ob mice than the WT group (P<0.05). In the ob/ob mice, no changes were found in diastolic left ventricular diameter (LVIDd), systolic left ventricular diameter (LVIDs), or ratio of velocity of early filling wave (E) to velocity of late filling wave due to atrial contraction (A) (P>0.05), or myocardial hypertrophy or collagen deposition, but notable decreases were found in short axis fractional shortening (FS) and ejection fraction (EF) (P<0.05), as well as the protein expression levels of PGC-1α and FNDC5 (P<0.05). DHM treatment for 16 weeks could significantly reverse the above changes in the ob/ob mice (P<0.05). Conclusion      DHM ameliorates cardiac function in the ob/ob mice, which might be related to PGC-1α/FNDC5 signalling pathway.

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Last Update: 2017-08-20