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Neuroprotective effect of liver X receptor agonist TO901317 on ganglion cells in alcohol-induced retinal injury in neonatal mice(PDF)

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

Issue:
2013年第05期
Page:
376-380
Research Field:
论著
Publishing date:

Info

Title:
Neuroprotective effect of liver X receptor agonist TO901317 on ganglion cells in alcohol-induced retinal injury in neonatal mice
Author(s):
Tang Yongping Yang Yang Xu Pei Xu Haiwei Yin Zhengqin
Center of Ophthalmology, Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Southwest Hospital, Department of Developmental Biology, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038, China
Keywords:
liver X receptor retina ganglion cell layer astrocyte
PACS:
R394.2; R595.6; R774.1
DOI:
-
Abstract:
Objective        To observe the effect of alcohol exposure on the retina of neonatal mice and the neuroprotective role of liver X receptor (LXR) agonist TO901317 (TO).       Methods        Mice were randomly divided into 3 groups, including a control group, an alcohol exposure group and a TO pretreatment group (n=3). Hematoxylin and eosin (HE) staining was used to observe the general structure of mouse retina, and immunochemistry was applied to detect NeuN-positive neurons and GFAP expression in the retina.       Results        LXRβ was mainly expressed in retinal ganglion cell layer of neonatal (postnatal day 6, P6) mice. HE staining showed a significant decrease in cell number in retinal ganglion cell layer of the alcohol exposure group compared with the control group (P<0.01), while TO could protect the ganglion cells from alcohol damage (P<0.05). Compared with the control group, NeuN-positive cells in the retinal ganglion cell layer of the alcohol exposure group significantly decreased (P<0.01), but the TO pretreatment group had more NeuN-positive cells than the alcohol exposure group (P<0.01). GFAP expression significantly increased in the retinal ganglion cell layer of the alcohol exposure group as compared with the control group and TO pretreatment group (P<0.01).       Conclusion        LXR agonist TO can inhibit alcohol-induced astrocyte activation in retina and rescue retinal ganglion cells in neonatal mice.

References:

唐永萍, 杨阳, 徐培, 等. TO901317修复酒精致新生小鼠视网膜节细胞损伤的实验研究[J].第三军医大学学报,2013,35(5):376-380.

Memo

Memo:
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Last Update: 2013-03-05